Histone H1T Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15574

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H1T Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 110-209 of mouse Histone H1T (NP_034507.2). LSKKAASGNDKGKGKKSASAKAKKMGLPRASRSPKSSKTKAVKKPKATPTKASGSGRKTKGAKGVQQRKSPAKARAANPNSGKAKMVMQKTDLRKAAGRK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H1T Antibody - Azide and BSA Free

Western Blot: Histone H1T AntibodyAzide and BSA Free [NBP3-15574]

Western Blot: Histone H1T AntibodyAzide and BSA Free [NBP3-15574]

Western Blot: Histone H1T Antibody [NBP3-15574] - Western blot analysis of extracts of various cell lines, using Histone H1T antibody (NBP3-15574) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: Histone H1T Antibody - Azide and BSA Free [NBP3-15574]

Immunohistochemistry: Histone H1T Antibody - Azide and BSA Free [NBP3-15574]

Immunohistochemistry: Histone H1T Antibody [NBP3-15574] - Immunofluorescence analysis of mouse testis using Histone H1T Rabbit pAb (NBP3-15574) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry: Histone H1T Antibody - Azide and BSA Free [NBP3-15574]

Immunohistochemistry: Histone H1T Antibody - Azide and BSA Free [NBP3-15574]

Immunohistochemistry: Histone H1T Antibody [NBP3-15574] - Immunofluorescence analysis of rat testis using Histone H1T Rabbit pAb (NBP3-15574) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Histone H1T Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H1T

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Histone H1t is a 207 amino acid long, 22 kDA protein encoded by the gene HIST1H1T that lacks polyA tails and instead obtain palindromic termination elements. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1T is found on chromosome 5. HIST1H1T has been investigated in childhood leukemia and hemochromatosis. It is involved in granzyme-A pathway, histone modification, and PKA signaling and is known to interact with various genes such as YWHAQ, YWHAZ, ACTA2, POU5F1, and GSK3B.

Alternate Names

dJ221C16.2, H1 histone family, member T (testis-specific), H1FTMGC163222, H1t, histone 1, H1t, histone cluster 1, H1t, histone H1t, Testicular H1 histone

Gene Symbol

H1-6

Additional Histone H1T Products

Product Documents for Histone H1T Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H1T Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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