Histone H2a [ac Lys9] Antibody (PS01-45)

Western Blot: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425]

Western Blot: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425] - Western blot analysis of Histone H2a on different lysates with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/1,000 dilution.

Lane 1: Hela whole cell lysate
Lane 2: Hela treated with 500 ng/ml TSA for 4 hours whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 3 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32425) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425]

Immunohistochemistry: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32425) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425]

Immunocytochemistry/ Immunofluorescence: Histone H2a [ac Lys9] Antibody (PS01-45) [NBP3-32425] - Immunocytochemistry analysis of NIH/3T3 cells labeling Histone H2a with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Histone H2a antibody (NBP3-32425) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.