Histone H2a [ubiquitylated Lys119] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62793
Key Product Details
Species Reactivity
Validated:
Human
Applications
Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Histone H2a [ubiquitylated Lys119] Antibody
Immunogen
Region of histone H2A containing the ubiquitinylated lysine 119 (H2AK119ub)
Modification
ubiquitylated Lys119
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2a [ubiquitylated Lys119] Antibody
![Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]](https://resources.bio-techne.com/images/products/Histone-H2a-[ubiquitylated-Lys119]-Antibody-Western-Blot-NBP2-62793-img0004.jpg "Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]")
Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]
Western Blot: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Analysis was performed on whole cell extracts from HeLa cells (25 ug, lane 1), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using the antibody against H2AK119ub.
Chromatin Immunoprecipitation (ChIP): Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - Assays were performed using human HeLa cells, the antibody against H2AK119ub and optimized PCR primer pairs for qPCR. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
![ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]](https://resources.bio-techne.com/images/products/Histone-H2a-[ubiquitylated-Lys119]-Antibody-ELISA-NBP2-62793-img0002.jpg "ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]")
ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793]
ELISA: Histone H2a [ubiquitylated Lys119] Antibody [NBP2-62793] - To determine the titer of the antibody, an analysis was performed using a serial dilution of the Diagenode antibody against H2AK119ub. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:23,000.Applications for Histone H2a [ubiquitylated Lys119] Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
2 - 5 ug
Dot Blot
1:20000
ELISA
1:200
Western Blot
1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS
Preservative
0.05% Sodium Azide and 0.05% ProClin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2A
Long Name
H2A Histone Family
Alternate Names
H2A1B, HIST1H2AB
Gene Symbol
H2AC20
Additional Histone H2A Products
Product Documents for Histone H2a [ubiquitylated Lys119] Antibody
Product Specific Notices for Histone H2a [ubiquitylated Lys119] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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