Histone H2AX Antibody (BLR059F)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14680

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14680
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR059F

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H2AX Antibodies »

Product Summary for Histone H2AX Antibody (BLR059F)

Immunogen

between 93 and 143 (C-term)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Histone H2AX Antibody (BLR059F)

Histone H2AX Antibody (BLR059F)

Western Blot;Histone H2AX Antibody (BLR059F)[NBP3-14680]

Western Blot;Histone H2AX Antibody (BLR059F) Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, RKO, LNCaP, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-H2AX recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin.

Applications for Histone H2AX Antibody (BLR059F)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Histone H2AX

Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.

Alternate Names

H2AFX

Gene Symbol

H2AX

Additional Histone H2AX Products

Product Documents for Histone H2AX Antibody (BLR059F)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Histone H2AX Antibody (BLR059F)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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