Histone H2AX Antibody - BSA Free, Novus Biologicals
Bio-Techne includes Novus Biologicals | Catalog # NBP3-03951
Discontinued Product
NBP3-03951 has been discontinued.
View all Histone H2AX products.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Histone H2AX Antibody - BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-143 of human Histone H2AX (NP_002096.1). VYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQEY
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Histone H2AX Antibody - BSA Free
Western Blot: Histone H2AX Antibody [NBP3-03951] - Analysis of extracts of various cell lines, using Histone H2Ax antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/Immunofluorescence: Histone H2AX Antibody [NBP3-03951] - Immunofluorescence analysis of U2OS cells using Histone H2AX Rabbit pAb (NBP3-03951) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Histone H2AX Antibody [NBP3-03951] - Rat brain using Histone H2AX antibody at dilution of 1:100 (40x lens).
Applications for Histone H2AX Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:100-1:200
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2AX
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody - BSA Free
Product Specific Notices for Histone H2AX Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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