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Histone H2A.Z [ac Lys4] Antibody (RM221)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61483

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-61483

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM221

Concentration

1 mg/ml

Product Summary for Histone H2A.Z [ac Lys4] Antibody (RM221)

Immunogen

An acetyl-peptide corresponding to Histone H2A.Z [ac Lys4]

Modification

ac Lys4

Specificity

This antibody reacts to Histone H2A.Z acetylated at Lysine 4. No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2A.Z [ac Lys4] Antibody (RM221)

Western Blot: Histone H2A.Z [ac Lys4] Antibody (RM221) [NBP2-61483] -

Western Blot: Histone H2A.Z [ac Lys4] Antibody (RM221) [NBP2-61483] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61483.
Immunocytochemistry/Immunofluorescence: Histone H2A.Z [ac Lys4] Antibody (RM221) [NBP2-61483] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61483, red). Actin filaments have been labeled with fluorescein phalloidin (green).
Multiplex Immunoassay: Histone H2A.Z [ac Lys4] Antibody (RM221) [NBP2-61483] - NBP2-61483 specifically reacts to Histone H2A.Z acetylated at Lysine 4 (K4ac). No cross reactivity with non-modified Lysine 4 or other acetylated Lysines in histone H2A.

Applications for Histone H2A.Z [ac Lys4] Antibody (RM221)

Application
Recommended Usage

ELISA

0.2 ug/ml - 1 ug/ml

Immunocytochemistry/ Immunofluorescence

1 ug/ml - 2 ug/ml

Multiplex Immunoassay

0.05 ug/ml - 0.5 ug/ml

Western Blot

0.5 ug/ml - 2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2A.Z

Alternate Names

H2A histone family, member Z, H2A.z, H2A/z, H2AZ histone, H2AZMGC117173, histone H2A.Z

Gene Symbol

H2AZ1

Additional Histone H2A.Z Products

Product Documents for Histone H2A.Z [ac Lys4] Antibody (RM221)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H2A.Z [ac Lys4] Antibody (RM221)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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