Histone H2A.Z Antibody

ELISA: Histone H2A.Z Antibody [NBP2-54618]

ELISA: H2AZ Antibody [NBP2-54618] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H2A.Z. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:87,500.

Immunofluorescence: Histone H2A.Z Antibody [NBP2-54618]

Immunofluorescence: H2AZ Antibody [NBP2-54618] - HeLa cells were stained with the antibody against H2A.Z. and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Top Figure: cells were immunofluorescently labeled with the H2A.Z antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. Middle Figure and lower Figure: staining of the cells with the H2A.Z antibody after incubation of the antibody with 10 ng/ul of the peptide used for immunisation of the rabbit (middle Figure) and with a peptide containing a sequence from the central part of the H2A.Z protein (lower Figure).