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Histone H2B Antibody (RM230) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26043

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-26043

Key Product Details

Species Reactivity

Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM230

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A peptide corresponding to the C-terminus of Human Histone H2B

Specificity

This antibody reacts to the Histone H2B protein, independent of post-translational modifications. No cross reactivity with other histone proteins.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2B Antibody (RM230) - Azide and BSA Free

Histone H2B Antibody (RM230) - Azide and BSA Free

Western Blot: Histone H2B Antibody (RM230) - Azide and BSA Free [NBP3-26043] -

Western Blot: Histone H2B Antibody (RM230) - Azide and BSA Free [NBP3-26043] - Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM230 at 0.2 ug/mL.
Histone H2B Antibody (RM230) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Histone H2B Antibody (RM230) - Azide and BSA Free [NBP3-26043] -

Immunocytochemistry/ Immunofluorescence: Histone H2B Antibody (RM230) - Azide and BSA Free [NBP3-26043] - Immunocytochemical staining of HeLa cells, using Clone RM230 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Applications for Histone H2B Antibody (RM230) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.2 ug/ml-1 ug/ml

Immunocytochemistry/ Immunofluorescence

0.5 ug/ml - 1 ug/ml

Multiplex Immunoassay

0.2 ug/ml - 1 ug/ml

Western Blot

0.1 ug/ml - 1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2B

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility tothe cellular machineries which require DNA as a template. Histones thereby play a central role in transcriptionregulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set ofpost-translational modifications of histones, also called histone code, and nucleosome remodeling

Long Name

H2B Histone Family

Alternate Names

HIST1H2BK

Gene Symbol

H2BC18

Additional Histone H2B Products

Product Documents for Histone H2B Antibody (RM230) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H2B Antibody (RM230) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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