Histone H2B Antibody

Western Blot: Histone H2B Antibody [NBP3-32426]

Western Blot: Histone H2B Antibody [NBP3-32426] - Western blot analysis of Histone H2B on different lysates with Rabbit anti-Histone H2B antibody (NBP3-32426) at 1/2,000 dilution.

Lane 1: MCF-7 cell lysate (10 ug/Lane)
Lane 2: Hela cell lysate (10 ug/Lane)
Lane 3: A549 cell lysate (10 ug/Lane)
Lane 4: 293T cell lysate (10 ug/Lane)
Lane 5: THP-1 cell lysate (10 ug/Lane)
Lane 6: K562 cell lysate (10 ug/Lane)
Lane 7: HL-60 cell lysate (10 ug/Lane)
Lane 8: NIH/3T3 cell lysate (10 ug/Lane)
Lane 9: Zebrafish tissue lysate (20 ug/Lane)
Lane 10: PC-12 cell lysate (10 ug/Lane)
Lane 11: RAW264.7 cell lysate (10 ug/Lane)

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 1 minute;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32426) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Histone H2B Antibody [NBP3-32426]

Immunohistochemistry: Histone H2B Antibody [NBP3-32426] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-Histone H2B antibody (NBP3-32426) at 1/3,400 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32426) at 1/3,400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.