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Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26029

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-26029

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM238

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

A phospho-peptide corresponding to Histone H2B type 1B [p Ser14]

Specificity

This antibody reacts to Histone H2B type 1B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free

Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free

Western Blot: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP3-26029] -

Western Blot: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP3-26029] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole, using RM238 at 0.5 ug/mL, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells.
Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP3-26029] -

Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP3-26029] - Immunocytochemistry of HeLa cells using Clone RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).

Applications for Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free

Application
Recommended Usage

ELISA

0.2 ug/mL-1 ug/mL

Immunocytochemistry/ Immunofluorescence

1 ug/ml - 2 ug/ml

Multiplex Immunoassay

0.1 ug/ml - 1 ug/ml

Western Blot

0.5 ug/ml-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H2B type 1B

The HIST1H2BB gene encodes a 126 amino acid long, 13 kDA histone H2B type 1-B that is critical in the nucleosome by functioning in transcription regulation, DNA replication and repair, as well as chromosomal stability. It participates in histone modification, apoptosis and authophagy, protein stability, RSF complex binding to the centromer, and signal transduction Activin A signaling regulation. HIST1H2BB is known to interact with genes EP300, ERCC6, DYRK1A, NFKB2, and TNFRSF1A. It has been researched regarding its role to lupus, immunodeficiency, cockayne syndrome, and hemochromatosis.

Alternate Names

H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804

Gene Symbol

H2BC3

Additional Histone H2B type 1B Products

Product Documents for Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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