Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26028
Recombinant Monoclonal Antibody
![Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26028_rabbit-histone-h2b-type-1b-mab-rm260-azide-and-bsa-free-22620242311095.png "Multiplex Immunoassay: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] -")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM260
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
An acetyl-peptide corresponding to Histone H2B type 1B [ac Lys23]
Specificity
This antibody reacts to Histone H2B type 1B acetylated at Lysine 23. No cross reactivity with other acetylated Lysines in histones.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free
Multiplex Immunoassay: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] -
Multiplex Immunoassay: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] - RM260 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in histone H2B.![Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26028_rabbit-histone-h2b-type-1b-mab-rm260-azide-and-bsa-free-246202413264.png "Western Blot: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] -")
Western Blot: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] -
Western Blot: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using RM260 at 0.5 ug/mL.![Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-26028_rabbit-histone-h2b-type-1b-mab-rm260-azide-and-bsa-free-24620241332411.png "Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] -")
Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] -
Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP3-26028] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM260 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free
Application
Recommended Usage
ELISA
0.2 ug/mL-1 ug/mL
Immunocytochemistry/ Immunofluorescence
0.5 ug/ml - 2 ug/ml
Multiplex Immunoassay
0.1 ug/ml - 0.5 ug/ml
Western Blot
0.5 ug/ml - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2B type 1B
Alternate Names
H2B histone family, member F, H2B/fHistone H2B.f, H2BFFH2B.1, histone 1, H2bb, histone cluster 1, H2bb, histone H2B type 1-B, Histone H2B.1, MGC119804
Gene Symbol
H2BC3
Additional Histone H2B type 1B Products
Product Documents for Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free
Product Specific Notices for Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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