Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26622

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26622-100ul
NBP3-26622-50ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6F6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H3.1 Antibodies »

Product Specifications

Immunogen

Synthesized peptide [UniProt P68431]

Modification

Dimethyl Lys 4

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

Western Blot: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] -

Western Blot: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Positive Western Blot detected in Mouse brain tissue, Mouse heart tissue.
All lanes: Histone H3.1 [Dimethyl Lys4] Antibody at 0.55ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 15 KDa
Observed band size: 15 KDa
Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Analysis of Histone H3.1 [Dimethyl Lys4] Antibody (6F6) diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] -

Immunocytochemistry/Immunofluorescence: Histone H3.1 [Dimethyl Lys4] Antibody (6F6) [NBP3-26622] - Staining of Hela cells with Histone H3.1 [Dimethyl Lys4] Antibody (6F6) at 1:34, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
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Applications for Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Histone H3.1

In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of Histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. Histone H3, the core protein of the nucleosome, becomes phosphorylated at the end of prophase. The two major sites of phosphorylation are the mitosis-specific site Ser 10 and Ser 28, both of which are extensively phosphorylated in DNA-bound forms of Histone H3 and in nucleosomal Histone H3. The nucleosome structure of Histone H3 promotes N-terminal phosphorylation in vitro. Phosphorylation of Histone H3 at Ser 10 is an essenti

Long Name

Histone Cluster 1 H3 Family Member A

Alternate Names

HIST1H3A

Gene Symbol

HIST1H3E

Additional Histone H3.1 Products

Product Documents for Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H3.1 [Dimethyl Lys4] Antibody (6F6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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