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Histone H3.3 [p Ser31] Antibody (SR1217)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21599

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21599-100ul
NBP3-21599-50ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1217

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Histone H3.3 [p Ser31] Antibody (SR1217)

Immunogen

A synthesized peptide derived from human phospho-Histone H3.3 [Ser31] (Uniprot #: P84243)

Modification

p Ser 31

Specificity

Detects endogenous levels of total phospho-Histone H3.3 [Ser31]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Histone H3.3 [p Ser31] Antibody (SR1217)

Western Blot: Histone H3.3 [p Ser 31] Antibody (SR1217) [NBP3-21599] -

Western Blot: Histone H3.3 [p Ser 31] Antibody (SR1217) [NBP3-21599] - Western blot analysis using NBP3-21599 in HeLa cell treated with Calyculin A lysate.

Applications for Histone H3.3 [p Ser31] Antibody (SR1217)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H3.3

Alternate Names

H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783

Gene Symbol

H3-3A

Additional Histone H3.3 Products

Product Documents for Histone H3.3 [p Ser31] Antibody (SR1217)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Histone H3.3 [p Ser31] Antibody (SR1217)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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