Histone H3.3 Antibody (ST50-08)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67530
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ST50-08
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human Histone H3.3 aa 50-100. (SwissProt: P84243 Human; SwissProt: P84244 Mouse; SwissProt: P84245 Rat)
Localization
Nucleus, Chromosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H3.3 Antibody (ST50-08)
Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530]
Western Blot: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Western blot analysis of Histone H3.3 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.Immunocytochemistry/ Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530]
Immunocytochemistry/Immunofluorescence: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530]
Immunohistochemistry-Paraffin: Histone H3.3 Antibody (ST50-08) [NBP2-67530] - Analysis of paraffin-embedded human placenta tissue with Rabbit anti-Histone H3.3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for Histone H3.3 Antibody (ST50-08)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H3.3
Alternate Names
H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783
Gene Symbol
H3-3A
Additional Histone H3.3 Products
Product Documents for Histone H3.3 Antibody (ST50-08)
Product Specific Notices for Histone H3.3 Antibody (ST50-08)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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