Histone H3.3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24697
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine, Chicken, Drosophila, Equine, Opossum, Zebrafish
Cited:
Avian - Chicken
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
A portion of amino acids 100-150 of human Histone H3.3 was used as the immunogen for the antibody.
Reactivity Notes
Ortangutan (100%), Salmon (100%).
Specificity
100 % homologous in human (Histone H3.3A, B, and C).
Marker
Marker of Transcriptionally Active Chromatin
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H3.3 Antibody
![Western Blot: Histone H3.3 Antibody [NBP2-24697]](https://resources.bio-techne.com/images/products/Histone-H3.3-Antibody-Western-Blot-NBP2-24697-img0003.jpg "Western Blot: Histone H3.3 Antibody [NBP2-24697]")
Western Blot: Histone H3.3 Antibody [NBP2-24697]
Western Blot: Histone H3.3 Antibody [NBP2-24697] - Total protein from human HeLa and A431 cells and mouse Neuro2A cells was separated on a 4-15% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-Histone H3.3 in blocking buffer with an anti-rabbit HRP secondary antibody using chemiluminescence.![Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]](https://resources.bio-techne.com/images/products/Histone-H3.3-Antibody-Immunohistochemistry-Paraffin-NBP2-24697-img0002.jpg "Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]")
Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697]
Immunohistochemistry-Paraffin: Histone H3.3 Antibody [NBP2-24697] - Human small intestine stained with Histone H3.3 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for Histone H3.3 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5-10 ug/ml
Western Blot
0.25-0.5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H3.3
Alternate Names
H3.3A, H3F3, H3F3A, Histone H3.3, Histone H3.3A, Histone H3.3B, MGC87782, MGC87783
Gene Symbol
H3-3A
Additional Histone H3.3 Products
Product Documents for Histone H3.3 Antibody
Product Specific Notices for Histone H3.3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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