Histone H3.3A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98769
![Western Blot: Histone H3.3A Antibody [NBP2-98769]](https://resources.bio-techne.com/images/products/Histone-H3.3A-Antibody-Western-Blot-NBP2-98769-img0002.jpg "Western Blot: Histone H3.3A Antibody [NBP2-98769]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Histone H3.3A Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human Histone H3.3A.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Histone H3.3A Antibody
Western Blot: Histone H3.3A Antibody [NBP2-98769]
Western Blot: Histone H3.3A Antibody [NBP2-98769] - Anti-Histone H3.3A rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 16 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769]](https://resources.bio-techne.com/images/products/Histone-H3.3A-Antibody-Immunoprecipitation-NBP2-98769-img0001.jpg "Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769]")
Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769]
Immunoprecipitation: Histone H3.3A Antibody [NBP2-98769] - Histone H3.3A was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate 2 ul anti-Histone H3.3A rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Histone H3.3A rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDaApplications for Histone H3.3A Antibody
Application
Recommended Usage
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Histone H3.3A
Alternate Names
H3 histone, family 3A, H3.3AH3F3H3F3B, H3.3B, histone H3.3, MGC87782, MGC87783
Gene Symbol
H3-3A
Additional Histone H3.3A Products
Product Documents for Histone H3.3A Antibody
Product Specific Notices for Histone H3.3A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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