Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [Janelia Fluor® 669]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30091JF669

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, C. elegans, Drosophila, Mammal, Yeast

Applications

Chromatin Immunoprecipitation (ChIP), Dot Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Janelia Fluor 669

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5E10-D8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone H4 Antibodies »

Product Summary for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [Janelia Fluor® 669]

Immunogen

Branched peptide found between amino acids 10-100 of Histone H4. [UniProt# P62805]

Reactivity Notes

Predicted to react all mammals

Modification

Monomethyl Lys20

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [Janelia Fluor® 669]

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Optimal dilutions of this antibody should be experimentally determined.

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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