Histone H4 [Monomethyl Arg3] Antibody (RM195)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61556
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Validated:
Human, Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM195
Concentration
1 mg/ml
Product Summary for Histone H4 [Monomethyl Arg3] Antibody (RM195)
Immunogen
A monomethyl -peptide corresponding to the Histone H4 [Monomethyl Arg3](RM195)
Modification
Methyl Arg3
Specificity
This antibody reacts to Histone H4 monomethylated at Arginine 3. No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [Monomethyl Arg3] Antibody (RM195)
Western Blot: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. using NBP2-61556, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - Immunocytochemical staining of HeLa cells using NBP2-61556 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Multiplex Immunoassay: Histone H4 [Monomethyl Arg3] Antibody (RM195) [NBP2-61556] - NBP2-61556 specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arg 3 (R3 ctrl), asymmetric dimethylated Arg 3 (R3me2a), symmetric dimethylated Arg 3 (R3me2s), or citrullinated Arg 3 (R3Cit) in Histone H4.
Applications for Histone H4 [Monomethyl Arg3] Antibody (RM195)
Application
Recommended Usage
ELISA
0.2 ug/mL - 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1 ug/ml - 2 ug/ml
Multiplex Immunoassay
0.2 ug/ml - 1 ug/ml
Western Blot
0.5 ug/ml-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4
Gene Symbol
H4C16
Additional Histone H4 Products
Product Documents for Histone H4 [Monomethyl Arg3] Antibody (RM195)
Product Specific Notices for Histone H4 [Monomethyl Arg3] Antibody (RM195)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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