Histone H4 [ac Lys5] Antibody (RM199)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61557

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Vertebrate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunoassay, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM199

Concentration

1 mg/ml

View all Histone H4 Antibodies »

Product Summary for Histone H4 [ac Lys5] Antibody (RM199)

Immunogen

An acetyl-peptide corresponding to the Histone H4 [ac Lys5]

Modification

ac Lys5

Specificity

This antibody reacts to Histone H4 acetylated at Lysine 5. No cross reactivity with other acetylated Lysines in Histone H4.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [ac Lys5] Antibody (RM199)

Western Blot: Histone H4 [ac Lys5] Antibody (RM199) [NBP2-61557] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, and (2) recombinant Histone H4. Using NBP2-61557, showed a band of Histone H4 acetylated at Lysine 5 in HeLa cells.
Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody (RM199) [NBP2-61557] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using NBP2-61557 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Multiplex Immunoassay: Histone H4 [ac Lys5] Antibody (RM199) [NBP2-61557] - NBP2-61557 specifically reacts to Histone H4 acetylated at Lysine 5 (K5ac). No cross reactivity with unmodified Lysine 5 (K5 ctrl), acetylated Lysine 8 (K8ac), Lysine 12 (K12ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.

Applications for Histone H4 [ac Lys5] Antibody (RM199)

Application
Recommended Usage

ELISA

0.2 ug/mL - 1 ug/mL

Immunocytochemistry/ Immunofluorescence

0.5 ug/ml - 2 ug/ml

Multiplex Immunoassay

0.05 ug/ml - 0.2 ug/Ml

Western Blot

0.2 ug/ml-1 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H4

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones. Methylation of position-specific lysine residues in histone N termini is a central modification for regulating epigenetic transitions in chromatin. Each methylatable lysine residue can exist in a mono, di, or tri methylated state. Arginine resdiues can also by mono or di methylated.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4

Gene Symbol

H4C16

Additional Histone H4 Products

Product Documents for Histone H4 [ac Lys5] Antibody (RM199)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone H4 [ac Lys5] Antibody (RM199)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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