Key Product Details

Species Reactivity

Validated:

Human, Mouse, C. elegans

Cited:

Human, Mouse

Predicted:

Chicken (100%), Drosophila (100%), Plant (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.53 mg/ml

View all Histone H4 Antibodies »

Product Specifications

Immunogen

Synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4 [Swiss Prot P62805].

Modification

Dimethyl Lys20

Localization

Nucleus; Chromosome

Marker

H4K20me2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H4 [Dimethyl Lys20] Antibody - BSA Free

Western Blot: Histone H4 [Dimethyl Lys20] AntibodyBSA Free [NB21-2089]

Western Blot: Histone H4 [Dimethyl Lys20] AntibodyBSA Free [NB21-2089]

Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.
Immunocytochemistry/ Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody - BSA Free [NB21-2089]

Immunocytochemistry/ Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody - BSA Free [NB21-2089]

Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).
Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.
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Applications for Histone H4 [Dimethyl Lys20] Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

2-5 ug per million cells

Dot Blot

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:2000
Application Notes
In Western blot, a band is seen at ~13kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

0.53 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H4

When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information.

Alternate Names

H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K20Me2

Entrez Gene IDs

121504 (Human); 320332 (Mouse)

Gene Symbol

H4-16

UniProt

P62805 (Human), P62806 (Mouse)

Additional Histone H4 Products

Product Documents for Histone H4 [Dimethyl Lys20] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone H4 [Dimethyl Lys20] Antibody - BSA Free

Epi-Plus antibody production in collaboration with Rockland Immunochemicals Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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