Histone H4 [ac Lys8] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42863
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Xenopus (100%). Backed by our 100% Guarantee.
Applications
Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys8 of human Histone H4. The exact sequence is proprietary.
Modification
ac Lys8
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Histone H4 [ac Lys8] Antibody
Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863]
Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of:A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).
Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863]
Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.Applications for Histone H4 [ac Lys8] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:5000
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Histone H4
Alternate Names
H4, H4/M, H4FM, H4M, HIST1H4I, HIST4H4, Histone Cluster 1 H4, Histone Cluster 1 H4i, Histone Cluster 4 H4, H4K8ac
Gene Symbol
H4C16
Additional Histone H4 Products
Product Documents for Histone H4 [ac Lys8] Antibody
Product Specific Notices for Histone H4 [ac Lys8] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.