Skip to main content

HIV-1 Gag p24 Antibody (8G9) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41337

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Virus

Applications

ELISA, Western Blot

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 8G9

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a recombinant full-length HIV-1 Gag p24 protein. Amino Acid Squence: pivqniqgqmvhqaisprtlnawvkvveekafspevipmfsalsegatpqdlntmlntvgghqaamqmlketineeaaewdrvhpvhagpiapgqmreprgsdiagttstlqeqigwmtnnppipvgeiykrwiilglnkivrmysptsildirqgpkepfrdyvdrfyktlraeqasqevknwmtetllvqnanpdcktilkalgpaatleemmtacqgvggpghkarvla

Specificity

By Western blot, anti-HIV-1 Gag p24 antibody detects a ~24 kDa, a ~41 kDa, and a ~55 kDa protein, corresponding to HIV-1 Gag p24 and to its precursors p41 and p55, respectively, in HIV-1 samples.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24, 41, 55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HIV-1 Gag p24 Antibody (8G9) [Biotin]

Western Blot: HIV-1 Gag p24 Antibody (8G9) [Biotin] [NBP2-41337] - Western blot analysis of 20 ng of recombinant HIV-1 Gag p24 protein with HIV-1 Gag p24 Antibody (8G9) [Biotin] at 0.2 ug/mL.
ELISA: HIV-1 Gag p24 Antibody (8G9) [Biotin] [NBP2-41337] - Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).

Applications for HIV-1 Gag p24 Antibody (8G9) [Biotin]

Application
Recommended Usage

ELISA

0.2 - 0.5 ug/mL

Western Blot

0.2-0.5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Antibody is supplied in PBS containing 1% BSA and 0.02% sodium azide.

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIV-1 Gag p24

The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 has been used as an integral part of multicomponent HIV-1 vaccines (3).

Long Name

Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa

Alternate Names

CA, Capsid Protein, HIV1 Gag p24, Pr55(Gag)

Gene Symbol

gag

Additional HIV-1 Gag p24 Products

Product Documents for HIV-1 Gag p24 Antibody (8G9) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HIV-1 Gag p24 Antibody (8G9) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...