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HIV-1 Tat specific factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06041

Novus Biologicals, part of Bio-Techne
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NBP1-06041

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QELYGDGKDGDTQTD corresponding to internal region of HIV-1 Tat specific factor 1 (near N-Terminus) according to NP_055315.2.

Epitope

C-QELYGDGKDGDTQTD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HIV-1 Tat specific factor 1 Antibody

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-06041]

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-06041]

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-06041] - (1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for HIV-1 Tat specific factor 1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 140 kDa band observed in Daudi lysates is reported in scientific literature (PMID: 8849451). An additional band of unknown identity was also consistently observed at 20 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HIV-1 Tat specific factor 1

Whereas most DNA sequence-specific transcription factors increase the rate of initiation and interact with enhancer or promoter DNA, human immunodeficiency virus-1 (HIV-1) Tat predominantly stimulates elongation and interacts with the trans-acting responsive (TAR) RNA element. Tat is essential for HIV replication.[supplied by OMIM]

Alternate Names

cofactor required for Tat activation of HIV-1 transcription, dJ196E23.2, HIV TAT specific factor 1, HIV Tat-specific factor 1, HIV-1 Tat specific factor 1, Tat-SF1

Entrez Gene IDs

27336 (Human); 317612 (Rat)

Gene Symbol

HTATSF1

Additional HIV-1 Tat specific factor 1 Products

Product Documents for HIV-1 Tat specific factor 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HIV-1 Tat specific factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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