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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 375 and 425 of human HIV-1 Tat specific factor 1 using the numbering given in entry NP_055315.2 (GeneID 27336).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIV-1 Tat specific factor 1 Antibody

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-18912]

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-18912]

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-18912] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg/ IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-18912 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP1-18912]

Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP1-18912]

Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP1-18912] - FFPE of human ovarian carcinoma. Affinity purified rabbit anti-Tat-SF1 dilution of 1:250.

Applications for HIV-1 Tat specific factor 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HIV-1 Tat specific factor 1

Whereas most DNA sequence-specific transcription factors increase the rate of initiation and interact with enhancer or promoter DNA, human immunodeficiency virus-1 (HIV-1) Tat predominantly stimulates elongation and interacts with the trans-acting responsive (TAR) RNA element. Tat is essential for HIV replication.[supplied by OMIM]

Alternate Names

cofactor required for Tat activation of HIV-1 transcription, dJ196E23.2, HIV TAT specific factor 1, HIV Tat-specific factor 1, HIV-1 Tat specific factor 1, Tat-SF1

Entrez Gene IDs

27336 (Human)

Gene Symbol

HTATSF1

Additional HIV-1 Tat specific factor 1 Products

Product Documents for HIV-1 Tat specific factor 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HIV-1 Tat specific factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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