HIV-1 Tat specific factor 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86966

Novus Biologicals, part of Bio-Techne
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NBP1-86966
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HIV-1 Tat specific factor 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant HIV-1 Tat specific factor 1 Protein corresponding to amino acids: GKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIATYQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGLPPDITVDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIV-1 Tat specific factor 1 Antibody

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-86966]

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-86966]

Western Blot: HIV-1 Tat specific factor 1 Antibody [NBP1-86966] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Adrenal Gland tissue.
Immunocytochemistry/ Immunofluorescence: HIV-1 Tat specific factor 1 Antibody [NBP1-86966]

Immunocytochemistry/ Immunofluorescence: HIV-1 Tat specific factor 1 Antibody [NBP1-86966]

Immunocytochemistry/Immunofluorescence: HIV-1 Tat specific factor 1 Antibody [NBP1-86966] - Staining of human cell line A-431 shows positivity in nuclei but not nucleoli.
Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP1-86966]

Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP1-86966]

Immunohistochemistry-Paraffin: HIV-1 Tat specific factor 1 Antibody [NBP1-86966] - Staining of human duodenum shows strong nuclear positivity in glandular cells.
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Applications for HIV-1 Tat specific factor 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIV-1 Tat specific factor 1

Whereas most DNA sequence-specific transcription factors increase the rate of initiation and interact with enhancer or promoter DNA, human immunodeficiency virus-1 (HIV-1) Tat predominantly stimulates elongation and interacts with the trans-acting responsive (TAR) RNA element. Tat is essential for HIV replication.[supplied by OMIM]

Alternate Names

cofactor required for Tat activation of HIV-1 transcription, dJ196E23.2, HIV TAT specific factor 1, HIV Tat-specific factor 1, HIV-1 Tat specific factor 1, Tat-SF1

Gene Symbol

HTATSF1

Additional HIV-1 Tat specific factor 1 Products

Product Documents for HIV-1 Tat specific factor 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HIV-1 Tat specific factor 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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