HLA A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14982

Novus Biologicals, part of Bio-Techne
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NBP2-14982
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLA A Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HLA A. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLA A Antibody

Western Blot: HLA A Antibody [NBP2-14982]

Western Blot: HLA A Antibody [NBP2-14982]

Western Blot: HLA A Antibody [NBP2-14982] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HLA-A antibody [N1C2] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: HLA A Antibody [NBP2-14982]

Immunohistochemistry-Paraffin: HLA A Antibody [NBP2-14982]

Immunohistochemistry-Paraffin: HLA A Antibody [NBP2-14982] - Human tonsil. HLA-A stained by HLA-A antibody [N1C2] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: HLA A Antibody [NBP2-14982]

Immunoprecipitation: HLA A Antibody [NBP2-14982]

Immunoprecipitation: HLA A Antibody [NBP2-14982] - IP samples: A549 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of HLA-A protein by 4 ug HLA-A antibody [N1C2] 10 % SDS-PAGE The immunoprecipitated HLA-A protein was detected by HLA-A antibody [N1C2] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for HLA A Antibody

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
IHC-P-Assay dependent.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA A

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]

Alternate Names

A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80

Gene Symbol

HLA-A

Additional HLA A Products

Product Documents for HLA A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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