HLA ABC Antibody (MHC-I/8147R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20573
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # MHC-I/8147R
Concentration
0.2 mg/ml
Product Summary for HLA ABC Antibody (MHC-I/8147R)
Immunogen
Recombinant fragment (around aa100-300) of human HLA-I protein (exact sequence is proprietary)
Localization
Cell Surface
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Human tonsil or lymph node.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for HLA ABC Antibody (MHC-I/8147R)
HLA ABC Antibody (MHC-I/8147R)
Formalin-fixed, paraffin-embedded human tonsil stained with HLA ABC antibody (MHC-I/8147R). Inset: PBS instead of primary antibody; secondary only negative control.
HLA ABC Antibody (MHC-I/8147R)
Formalin-fixed, paraffin-embedded human tonsil stained with HLA ABC antibody (MHC-I/8147R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.Applications for HLA ABC Antibody (MHC-I/8147R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HLA ABC
Alternate Names
A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80
Gene Symbol
HLA-A
Additional HLA ABC Products
Product Documents for HLA ABC Antibody (MHC-I/8147R)
Product Specific Notices for HLA ABC Antibody (MHC-I/8147R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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