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HLA ABC Antibody (MHC-I/8366R)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20580

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MHC-I/8366R

Concentration

0.2 mg/ml

Product Summary for HLA ABC Antibody (MHC-I/8366R)

Immunogen

Recombinant fragment (around aa100-300) of human HLA-I protein (exact sequence is proprietary)

Localization

Cell Surface

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Human tonsil or lymph node.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for HLA ABC Antibody (MHC-I/8366R)

HLA ABC Antibody (MHC-I/8366R)

HLA ABC Antibody (MHC-I/8366R)

Formalin-fixed, paraffin-embedded human tonsil stained with HLA ABC antibody (MHC-I/8366R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications for HLA ABC Antibody (MHC-I/8366R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLA ABC

HLA CLass I is involved in the presentation of foreign antigens to the immune system. HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC.

Alternate Names

A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80

Gene Symbol

HLA-A

Additional HLA ABC Products

Product Documents for HLA ABC Antibody (MHC-I/8366R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA ABC Antibody (MHC-I/8366R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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