HLA ABC Antibody (YTH862.2)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65411
![Flow Cytometry: HLA ABC Antibody (YTH862.2) - BSA Free [NB100-65411]](https://resources.bio-techne.com/images/products/HLA-ABC-Antibody-YTH862-2-Flow-Cytometry-NB100-65411-img0001.jpg "Flow Cytometry: HLA ABC Antibody (YTH862.2) - BSA Free [NB100-65411]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # YTH862.2
Concentration
1.0 mg/ml
Product Specifications
Immunogen
PHA activated human peripheral blood lymphocytes
Specificity
NB100-65411 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. MHC Class I is expressed by all nucleated cells. Recent studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells (1).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for HLA ABC Antibody (YTH862.2)
Flow Cytometry: HLA ABC Antibody (YTH862.2) - BSA Free [NB100-65411]
Flow Cytometry: HLA ABC Antibody (YTH862.2) [NB100-65411] - Staining of human peripheral blood lymphocytes.Applications for HLA ABC Antibody (YTH862.2)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HLA ABC
Alternate Names
A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80
Gene Symbol
HLA-A
Additional HLA ABC Products
Product Documents for HLA ABC Antibody (YTH862.2)
Product Specific Notices for HLA ABC Antibody (YTH862.2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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