HLA DQA1 Antibody (7W0M7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15364

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15364-20ul
NBP3-15364-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7W0M7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HLA DQA1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 155-254 of human HLA DQA1 (P01909). EGVSETSFLSKSDHSFFKISYLTLLPSAEESYDCKVEHWGLDKPLLKHWEPEIPAPMSELTETVVCALGLSVGLVGIVVGTVFIIRGLRSVGASRHQGPL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLA DQA1 Antibody (7W0M7)

Western Blot: HLA DQA1 Antibody (7W0M7) [NBP3-15364]

Western Blot: HLA DQA1 Antibody (7W0M7) [NBP3-15364]

Western Blot: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Western blot analysis of extracts of various cell lines, using HLA DQA1 Rabbit mAb (NBP3-15364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364]

Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364]

Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Immunohistochemistry of paraffin-embedded mouse spleen using HLA DQA1 Rabbit mAb (NBP3-15364) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364]

Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364]

Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Immunohistochemistry of paraffin-embedded rat spleen using HLA DQA1 Rabbit mAb (NBP3-15364) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for HLA DQA1 Antibody (7W0M7)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLA DQA1

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]

Alternate Names

CD, CELIAC1DQ alpha 1 chain, DC-1 alpha chain, DQ-A1, FLJ27088, FLJ27328, GSE, HLA class II histocompatibility antigen, DQ(W3) alpha chain, HLA-DCA, HLA-DQA, leucocyte antigen DQA1, leukocyte antigen alpha chain, major histocompatibility complex, class II, DQ alpha 1, MGC149527, MHC class II antigen, MHC class II DQA1, MHC class II HLA-D alpha glycoprotein, MHC class II HLA-DQ-alpha-1, MHC class II surface glycoprotein, MHC HLA-DQ alpha

Gene Symbol

HLA-DQA1

Additional HLA DQA1 Products

Product Documents for HLA DQA1 Antibody (7W0M7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DQA1 Antibody (7W0M7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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