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HLA DQA1 Antibody (HL2139) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25507

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25507

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2139

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human HLA DQA1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLA DQA1 Antibody (HL2139) - Azide and BSA Free

HLA DQA1 Antibody (HL2139) - Azide and BSA Free

Western Blot: HLA DQA1 Antibody (HL2139) - Azide and BSA Free [NBP3-25507] -

Western Blot: HLA DQA1 Antibody (HL2139) - Azide and BSA Free [NBP3-25507] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DQA1 antibody [HL2139] (NBP3-25507) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
HLA DQA1 Antibody (HL2139) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: HLA DQA1 Antibody (HL2139) - Azide and BSA Free [NBP3-25507] -

Immunocytochemistry/Immunofluorescence: HLA DQA1 Antibody (HL2139) - Azide and BSA Free [NBP3-25507] - HLA-DQA1 antibody [HL2139] detects HLA-DQA1 protein at cell membrane by immunofluorescent analysis. Sample: Raji cells were fixed in ice-cold MeOH for 5 min. Green: HLA-DQA1 stained by HLA-DQA1 antibody [HL2139] (NBP3-25507) diluted at 1:500. Blue: Fluoroshield with DAPI .

Applications for HLA DQA1 Antibody (HL2139) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLA DQA1

HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq]

Alternate Names

CD, CELIAC1DQ alpha 1 chain, DC-1 alpha chain, DQ-A1, FLJ27088, FLJ27328, GSE, HLA class II histocompatibility antigen, DQ(W3) alpha chain, HLA-DCA, HLA-DQA, leucocyte antigen DQA1, leukocyte antigen alpha chain, major histocompatibility complex, class II, DQ alpha 1, MGC149527, MHC class II antigen, MHC class II DQA1, MHC class II HLA-D alpha glycoprotein, MHC class II HLA-DQ-alpha-1, MHC class II surface glycoprotein, MHC HLA-DQ alpha

Gene Symbol

HLA-DQA1

Additional HLA DQA1 Products

Product Documents for HLA DQA1 Antibody (HL2139) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA DQA1 Antibody (HL2139) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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