HLA DR/DP Antibody (MEM-136) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-447

Novus Biologicals, part of Bio-Techne
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NB500-447
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-136

Format

BSA Free

Concentration

1.0 mg/ml

View all HLA DR/DP Antibodies »

Product Specifications

Immunogen

PHA-activated peripheral blood lymphocytes.

Specificity

The antibody MEM-136 recognizes common epitope on beta-chain of human HLA-DR and HLA-DP. It reacts with alpha/beta dimer as well as with dissociated beta-subunit. DR and DP are the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC; dendritic cells, B lymphocytes, monocytes, macrophages).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HLA DR/DP Antibody (MEM-136) - BSA Free

Western Blot: HLA DR/DP Antibody (MEM-136)BSA Free [NB500-447]

Western Blot: HLA DR/DP Antibody (MEM-136)BSA Free [NB500-447]

Western Blot: HLA DR DP Antibody (MEM-136) [NB500-447] - Fig. 1. Western Blotting analysis of MHC Class II in whole cell lysate of RAJI human Burkitt lymphoma cell line using anti-human HLA-DR+DP (MEM-136). The antibody MEM-136 recognizes beta-chain of HLA-DR antigen under non-reducing conditions. Lane 1: immunostaining with anti-human HLA-DR+DP (MEM-136) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).

Applications for HLA DR/DP Antibody (MEM-136) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blot - Non-reducing conditions. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLA DR/DP

Human MHC class II antigens are transmembrane glycoproteins composed of an alpha (36 kDa) and a beta chain (27kDa) and are expressed primarily on antigen presenting cells. Human MHC class II genes are located in the HLA-D region that encodes at least 6 alpha and 10 beta chain genes. Three loci, DR, DQ and DP, encode the major products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells and therefore have a critical role in the initiation of the immune response. It has been shown that some autoimmune diseases are associated with certain class II alleles.

Alternate Names

FLJ51114, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, major histocompatibility complex, class II, DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA, MLRW

Entrez Gene IDs

3122 (Human)

Gene Symbol

HLA-DRA

Additional HLA DR/DP Products

Product Documents for HLA DR/DP Antibody (MEM-136) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLA DR/DP Antibody (MEM-136) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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