HLA DRA Antibody (169-1B5.2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45043

Novus Biologicals, part of Bio-Techne
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NBP2-45043-0.1mg
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Key Product Details

Species Reactivity

Human, Bovine, Feline

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 169-1B5.2

Concentration

0.2 mg/ml

View all HLA DRA Antibodies »

Product Specifications

Immunogen

Human peripheral blood leukocytes (Uniprot: P01903)

Localization

Cell surface

Specificity

This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47722)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HLA DRA Antibody (169-1B5.2)

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043]

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043]

Flow Cytometry: HLA DRA Antibody (169-1B5.2) [NBP2-45043] - Flow Cytometric Analysis of PFA-fixed Raji cells. HLA DRA Antibody (169-1B5.2) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for HLA DRA Antibody (169-1B5.2)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HLA DRA

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Alternate Names

FLJ51114, histocompatibility antigen HLA-DR alpha, HLA class II histocompatibility antigen, DR alpha chain, HLA-DRA1, major histocompatibility complex, class II, DR alpha, MHC cell surface glycoprotein, MHC class II antigen DRA, MLRW

Gene Symbol

HLA-DRA

UniProt

P01903

Additional HLA DRA Products

Product Documents for HLA DRA Antibody (169-1B5.2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HLA DRA Antibody (169-1B5.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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