HLA DRB1 Antibody (JE60-43)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32433
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE60-43
Concentration
1 mg/ml
Product Summary for HLA DRB1 Antibody (JE60-43)
Immunogen
Synthetic peptide within Human HLA DRB1 aa 151-200 / 266. (Uniprot: P01911)
Localization
Cell membrane. Endoplasmic reticulum.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HLA DRB1 Antibody (JE60-43)
Western Blot: HLA DRB1 Antibody (JE60-43) [NBP3-32433]
Western Blot: HLA DRB1 Antibody (JE60-43) [NBP3-32433] - Western blot analysis of HLA DRB1 on different lysates with Rabbit anti-HLA DRB1 antibody (NBP3-32433) at 1/1,000 dilution.Lane 1: Ramos cell lysate
Lane 2: Raji cell lysate
Lane 3: Daudi cell lysate
Lane 4: HDLM-2 cell lysate
Lane 5: A375 cell lysate
Lane 6: HeLa cell lysate (negative)
Lysates/proteins at 20 ug/Lane.
Predicted band size: 29 kDa
Observed band size: 29 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32433) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: HLA DRB1 Antibody (JE60-43) [NBP3-32433]
Immunohistochemistry: HLA DRB1 Antibody (JE60-43) [NBP3-32433] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-HLA DRB1 antibody (NBP3-32433) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32433) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry: HLA DRB1 Antibody (JE60-43) [NBP3-32433]
Immunohistochemistry: HLA DRB1 Antibody (JE60-43) [NBP3-32433] - Immunofluorescence analysis of paraffin-embedded human skin tissue labeling HLA DRB1 with Rabbit anti-HLA DRB1 antibody (NBP3-32433) at 1/100 dilution.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32433, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Applications for HLA DRB1 Antibody (JE60-43)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HLA DRB1
Alternate Names
Clone P2-beta-3, DR1, DR-1, DR12, DR-12, DR13, DR-13, DR14, DR-14, DR16, DR-16, DR4, DR-4, DR5, DR-5, DR7, DR-7, DR8, DR-8, DR9, DR-9, DRB1, DRw10, DRw11, DRw8, DW2.2/DR2.2, FLJ75017, FLJ76359, HLA class II antigen beta chain, HLA class II histocompatibility antigen, DR-1 beta chain, HLA-DR1B, HLA-DRB, HLA-DRB1*, HLA-DRB2, HLA-DR-beta 1, human leucocyte antigen DRB1, leucocyte antigen DR beta 1 chain, leucocyte antigen DRB1, lymphocyte antigen DRB1, major histocompatibility complex, class II, DR beta 1, MHC class II antigen DRB1*1, MHC class II antigen DRB1*10, MHC class II antigen DRB1*11, MHC class II antigen DRB1*12, MHC class II antigen DRB1*13, MHC class II antigen DRB1*14, MHC class II antigen DRB1*15, MHC class II antigen DRB1*16, MHC class II antigen DRB1*3, MHC class II antigen DRB1*4, MHC class II antigen DRB1*7, MHC class II antigen DRB1*8, MHC class II antigen DRB1*9, MHC class II antigen HLA-DR13, MHC class II HLA-DR beta 1 chain, MHC class II HLA-DR-beta cell surface glycoprotein, MHC class II HLA-DRw10-beta, SS1
Gene Symbol
HLA-DRB1
Additional HLA DRB1 Products
Product Documents for HLA DRB1 Antibody (JE60-43)
Product Specific Notices for HLA DRB1 Antibody (JE60-43)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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