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HLA DRB1 Antibody (SPM423)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45313

Novus Biologicals, part of Bio-Techne
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NBP2-45313-0.02mg
NBP2-45313-0.1mg

Key Product Details

Species Reactivity

Human, Monkey, Mouse (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # SPM423

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Activated human peripheral blood mononuclear cells

Reactivity Notes

Does not react with Mouse.

Localization

Cell Surface

Specificity

This monoclonal antibody reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. The L243 antibody recognizes a different epitope than the LN3 monoclonal antibody, and these antibodies do not cross-block binding to each others respective epitopes. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11552)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HLA DRB1 Antibody (SPM423)

Western Blot: HLA DRB1 Antibody (SPM423) [NBP2-45313]

Western Blot: HLA DRB1 Antibody (SPM423) [NBP2-45313]

Western Blot: HLA DRB1 Antibody (SPM423) [NBP2-45313] - Western Blot Analysis of human Spleen tissue lysate using HLA DRB1 Antibody (SPM423).
Immunocytochemistry/ Immunofluorescence: HLA DRB1 Antibody (SPM423) [NBP2-45313]

Immunocytochemistry/ Immunofluorescence: HLA DRB1 Antibody (SPM423) [NBP2-45313]

Immunocytochemistry/Immunofluorescence: HLA DRB1 Antibody (SPM423) [NBP2-45313] - ICC/IF analysis of Raji cells labeling HLA-DR with conjugated with CF594 (Green). The nuclear counterstain is Red Dot (Red)
Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM423) [NBP2-45313]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM423) [NBP2-45313]

Immunohistochemistry-Paraffin: HLA DRB1 Antibody (SPM423) [NBP2-45313] - Human Histiocytoma stained with HLA-DRB Monoclonal Antibody (SPM423).

Applications for HLA DRB1 Antibody (SPM423)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HLA-DRB1

HLA DRB1 can be used for HLA typing.

Long Name

Major histocompatibility complex, class II, DR beta 1

Alternate Names

DRB1, HLA-DR1B, HLA-DRB, SS1

Gene Symbol

HLA-DRB1

UniProt

Additional HLA-DRB1 Products

Product Documents for HLA DRB1 Antibody (SPM423)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA DRB1 Antibody (SPM423)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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