HLA-E Antibody (MEM-E/07) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-310
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-E/07
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
Localization
Type I membrane protein.
Specificity
The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HLA-E Antibody (MEM-E/07) - BSA Free
Immunohistochemistry: HLA-E Antibody (MEM-E/07) - BSA Free [NB500-310]
Immunohistochemistry: MHC Class I Antibody (MEM-E/07) [NB500-310] - staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07).Applications for HLA-E Antibody (MEM-E/07) - BSA Free
Application
Recommended Usage
Flow Cytometry
1-4 ug/ml
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
10 ug/ml
Immunoprecipitation
1:50
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HLA-E
Long Name
Major Histocompatibility Complex, Class I, E
Alternate Names
HLA-6.2, HLAE, QA1
Entrez Gene IDs
3133 (Human)
Gene Symbol
HLA-E
Additional HLA-E Products
Product Documents for HLA-E Antibody (MEM-E/07) - BSA Free
Product Specific Notices for HLA-E Antibody (MEM-E/07) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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