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HLA-E Antibody (MEM-E/07) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-310

Novus Biologicals, part of Bio-Techne
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NB500-310

Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-E/07

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for HLA-E Antibody (MEM-E/07) - BSA Free

Immunogen

Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

Localization

Type I membrane protein.

Specificity

The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HLA-E Antibody (MEM-E/07) - BSA Free

Immunohistochemistry: HLA-E Antibody (MEM-E/07) - BSA Free [NB500-310]

Immunohistochemistry: HLA-E Antibody (MEM-E/07) - BSA Free [NB500-310]

Immunohistochemistry: MHC Class I Antibody (MEM-E/07) [NB500-310] - staining of human spleen (paraffin sections) with anti-HLA-E (clone MEM-E/07).

Applications for HLA-E Antibody (MEM-E/07) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLA-E

HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules.

Long Name

Major Histocompatibility Complex, Class I, E

Alternate Names

HLA-6.2, HLAE, QA1

Entrez Gene IDs

3133 (Human)

Gene Symbol

HLA-E

Additional HLA-E Products

Product Documents for HLA-E Antibody (MEM-E/07) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HLA-E Antibody (MEM-E/07) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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