HLA-E Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14985

Novus Biologicals, part of Bio-Techne
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NBP2-14985
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLA-E Antibodies »

Product Summary for HLA-E Antibody

Immunogen

Full length human HLA-E Recombinant protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLA-E Antibody

Western Blot: HLA-E Antibody [NBP2-14985]

Western Blot: HLA-E Antibody [NBP2-14985]

Western Blot: HLA-E Antibody [NBP2-14985] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: THP-1 D: HL-60 10% SDS PAGE; antibody diluted at 1:10000.
Immunohistochemistry-Paraffin: HLA-E Antibody [NBP2-14985]

Immunohistochemistry-Paraffin: HLA-E Antibody [NBP2-14985]

Immunohistochemistry-Paraffin: HLA-E Antibody [NBP2-14985] - U87 xenograft, using HLA-E antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for HLA-E Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HLA-E

HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq]

Long Name

Major Histocompatibility Complex, Class I, E

Alternate Names

HLA-6.2, HLAE, QA1

Gene Symbol

HLA-E

UniProt

P13747 (Human)

Additional HLA-E Products

Product Documents for HLA-E Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HLA-E Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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