HLCS Antibody (OTI1E4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70893

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HLCS Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HLCS(NP_000402) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

80.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HLCS Antibody (OTI1E4) - Azide and BSA Free

Western Blot: HLCS Antibody (OTI1E4)Azide and BSA Free [NBP2-70893]

Western Blot: HLCS Antibody (OTI1E4)Azide and BSA Free [NBP2-70893]

Western Blot: HLCS Antibody (OTI1E4) - Azide and BSA Free [NBP2-70893] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLCS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLCS.
Immunohistochemistry: HLCS Antibody (OTI1E4) - Azide and BSA Free [NBP2-70893]

Immunohistochemistry: HLCS Antibody (OTI1E4) - Azide and BSA Free [NBP2-70893]

Immunohistochemistry: HLCS Antibody (OTI1E4) - Azide and BSA Free [NBP2-70893] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HLCS mouse monoclonal antibody.
Flow Cytometry: HLCS Antibody (OTI1E4) - Azide and BSA Free [NBP2-70893]

Flow Cytometry: HLCS Antibody (OTI1E4) - Azide and BSA Free [NBP2-70893]

Flow Cytometry: HLCS Antibody (OTI1E4) - Azide and BSA Free [NBP2-70893] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-HLCS antibody, and then analyzed by flow cytometry.
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Applications for HLCS Antibody (OTI1E4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HLCS

Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA; MIM 232000), pyruvate carboxylase (PC; MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1; MIM 609010), and acetyl-CoA carboxylase (ACACA; MIM 200350).[supplied by OMIM]

Alternate Names

Biotin apo-protein ligase, biotin--protein ligase, EC 6.3.4.-, HCSholocarboxylase synthetase, EC 6.3.410holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)] ligase)

Gene Symbol

HLCS

Additional HLCS Products

Product Documents for HLCS Antibody (OTI1E4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HLCS Antibody (OTI1E4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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