HLCS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81759

Novus Biologicals, part of Bio-Techne
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NBP1-81759
NBP1-81759-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HLCS Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DLPYDYSSSLESVADETSPEREGRRVNLTGKAPNILLYVGSDSQEALGRFHEVRSVLADCVDIDSYILYHLLEDSALRDPWTDNCLLLVIATRESIPEDLYQKFMAYLSQGGKVLGLSSSFTFGGFQV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLCS Antibody

Immunocytochemistry/ Immunofluorescence: HLCS Antibody [NBP1-81759]

Immunocytochemistry/ Immunofluorescence: HLCS Antibody [NBP1-81759]

Immunocytochemistry/Immunofluorescence: HLCS Antibody [NBP1-81759] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: HLCS Antibody [NBP1-81759]

Immunohistochemistry-Paraffin: HLCS Antibody [NBP1-81759]

Immunohistochemistry-Paraffin: HLCS Antibody [NBP1-81759] - Staining of human kidney shows granular cytoplasmic positivity in tubule cells.

Applications for HLCS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 21214190). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HLCS

Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA; MIM 232000), pyruvate carboxylase (PC; MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1; MIM 609010), and acetyl-CoA carboxylase (ACACA; MIM 200350).[supplied by OMIM]

Alternate Names

Biotin apo-protein ligase, biotin--protein ligase, EC 6.3.4.-, HCSholocarboxylase synthetase, EC 6.3.410holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)] ligase)

Gene Symbol

HLCS

Additional HLCS Products

Product Documents for HLCS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HLCS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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