Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HLCS Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 to 50 of human Holocarboxylase Synthetase using the numbering given in entry NP_001229714.1 (GeneID 3141).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HLCS Antibody

Western Blot: HLCS Antibody [NBP2-32174]

Western Blot: HLCS Antibody [NBP2-32174]

Western Blot: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: HLCS Antibody [NBP2-32174]

Immunoprecipitation: HLCS Antibody [NBP2-32174]

Immunoprecipitation: HLCS Antibody [NBP2-32174] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HLCS antibody NBP2-32174 used for IP at 6 ug per reaction. HLCS was also immunoprecipitated by rabbit anti-HLCS antibody BL15481. For blotting immunoprecipitated HLCS, NBP2-32174 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for HLCS Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HLCS

Holocarboxylase synthetase (EC 6.3.4.10) covalently links biotin to propionyl-CoA carboxylase (PCCA; MIM 232000), pyruvate carboxylase (PC; MIM 608786), alpha-methylcrotonyl-CoA carboxylase (MCCC1; MIM 609010), and acetyl-CoA carboxylase (ACACA; MIM 200350).[supplied by OMIM]

Alternate Names

Biotin apo-protein ligase, biotin--protein ligase, EC 6.3.4.-, HCSholocarboxylase synthetase, EC 6.3.410holocarboxylase synthetase (biotin-(proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-(proprionyl-CoA-carboxylase(ATP-hydrolysing)) ligase), holocarboxylase synthetase (biotin-[proprionyl-Coenzyme A-carboxylase(ATP-hydrolysing)] ligase)

Gene Symbol

HLCS

UniProt

NP_001229714.1 (Human)

Additional HLCS Products

Product Documents for HLCS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HLCS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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