HLX1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89719
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CSFPLDPAAVKKPSFCIADILHAGVGDLGAAPEGLAGASAAALTAHLGSVHPHASFQAAARSPLRPTPVVAPSEVPAGFPQRLSPLPAAYHHHHPQQQQQQQ
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%). Human reactivity reported in scientific literature (PMID: 23455154).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HLX1 Antibody
![Immunocytochemistry/ Immunofluorescence: HLX1 Antibody [NBP1-89719]](https://resources.bio-techne.com/images/products/HLX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89719-img0006.jpg "Immunocytochemistry/ Immunofluorescence: HLX1 Antibody [NBP1-89719]")
Immunocytochemistry/ Immunofluorescence: HLX1 Antibody [NBP1-89719]
Immunocytochemistry/Immunofluorescence: HLX1 Antibody [NBP1-89719] - Staining of human cell line HEK 293 shows positivity in nucleus and cytoplasm. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: HLX1 Antibody [NBP1-89719]](https://resources.bio-techne.com/images/products/HLX1-Antibody-Immunohistochemistry-Paraffin-NBP1-89719-img0002.jpg "Immunohistochemistry-Paraffin: HLX1 Antibody [NBP1-89719]")
Immunohistochemistry-Paraffin: HLX1 Antibody [NBP1-89719]
Immunohistochemistry-Paraffin: HLX1 Antibody [NBP1-89719] - Staining of human tonsil shows strong nuclear positivity in reaction center cells.Applications for HLX1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. WB reported in the literature (PMID: 29581848).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HLX1
Alternate Names
H2.0 (Drosophila)-like homeo box 1, H2.0-like homeo box 1, H2.0-like homeo box-1, H2.0-like homeobox, H2.0-like homeobox 1, H2.0-like homeobox protein, HB24, HLX1H2.0-like homeobox 1 (Drosophila), Homeobox protein HB24, Homeobox protein HLX1
Gene Symbol
HLX
Additional HLX1 Products
Product Documents for HLX1 Antibody
Product Specific Notices for HLX1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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