HMBOX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31316

Novus Biologicals, part of Bio-Techne
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NBP1-31316
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Chicken (97%), Mouse (98%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMBOX1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMBOX1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMBOX1 Antibody

Western Blot: HMBOX1 Antibody [NBP1-31316]

Western Blot: HMBOX1 Antibody [NBP1-31316]

Western Blot: HMBOX1 Antibody [NBP1-31316] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HMBOX1 Antibody [NBP1-31316]

Immunocytochemistry/ Immunofluorescence: HMBOX1 Antibody [NBP1-31316]

Immunocytochemistry/Immunofluorescence: HMBOX1 Antibody [NBP1-31316] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMBOX1 protein stained by HMBOX1 antibody [N3C3] (NBP1-31316) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (NBP1-33273) diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: HMBOX1 Antibody [NBP1-31316]

Immunohistochemistry-Paraffin: HMBOX1 Antibody [NBP1-31316]

Immunohistochemistry-Paraffin: HMBOX1 Antibody [NBP1-31316] - SW480 xenograft , using Homeobox-containing protein 1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Applications for HMBOX1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IP-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMBOX1

HMBOX1, also known as Homeobox-containing protein 1, is a 47kDa 429 amino acid protein with four isoforms produced by alternative splicing. The gene can be found in the nucleus and the cytoplasm where it acts as a transcriptional repressor. Current research on HMBOX1 is being conducted in relation to bronchopulmonary dysplasia, immunodeficiency and prostatitis. HMBOX1 is linked to the MRNA splicing, nuclear transport, stem cell differentiation, tube formation and transport pathways where it interacts with SUMO2 and ENTPD2.

Alternate Names

FLJ21616, HNF1LA, homeobox containing 1, homeobox-containing protein 1, homeobox-containing protein PBHNF, PBHNF

Gene Symbol

HMBOX1

Additional HMBOX1 Products

Product Documents for HMBOX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HMBOX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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