HMG20A Antibody (4D5)
Novus Biologicals, part of Bio-Techne | Catalog # H00010363-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 4D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HMG20A (NP_060670.1, 153 a.a. ~ 262 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDEADRDKERYMKELEQYQKTEAYKVFSRKTQDRQKGKSHRQDAARQATHDHEKETEVKERSVFDIPIFTEEFLNHSKAREAELRQLRKSNMEFEERNAALQKHVESMRT
Specificity
Reacts with high-mobility group 20A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for HMG20A Antibody (4D5)
![Western Blot: HMG20A Antibody (4D5) [H00010363-M01]](https://resources.bio-techne.com/images/products/HMG20A-Antibody-4D5-Western-Blot-H00010363-M01-img0004.jpg "Western Blot: HMG20A Antibody (4D5) [H00010363-M01]")
Western Blot: HMG20A Antibody (4D5) [H00010363-M01]
Western Blot: HMG20A Antibody (4D5) [H00010363-M01] - Analysis of HMG20A expression in transfected 293T cell line by HMG20A monoclonal antibody (M01), clone 4D5.Lane 1: HMG20A transfected lysate(40.1 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: HMG20A Antibody (4D5) [H00010363-M01]](https://resources.bio-techne.com/images/products/HMG20A-Antibody-4D5-Immunohistochemistry-Paraffin-H00010363-M01-img0002.jpg "Immunohistochemistry-Paraffin: HMG20A Antibody (4D5) [H00010363-M01]")
Immunohistochemistry-Paraffin: HMG20A Antibody (4D5) [H00010363-M01]
Immunohistochemistry-Paraffin: HMG20A Antibody (4D5) [H00010363-M01] - Analysis of monoclonal antibody to HMG20A on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: HMG20A Antibody (4D5) [H00010363-M01] - Detection limit for recombinant GST tagged HMG20A is 0.03 ng/ml as a capture antibody.
Applications for HMG20A Antibody (4D5)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HMG20A
Alternate Names
FLJ10739, high-mobility group 20A, HMG box domain containing 1, HMG box-containing protein 20A, HMG domain-containing protein 1, HMGX1HMG domain-containing protein HMGX1, HMGXB1high mobility group protein 20A
Entrez Gene IDs
10363 (Human)
Gene Symbol
HMG20A
UniProt
Additional HMG20A Products
Product Documents for HMG20A Antibody (4D5)
Product Specific Notices for HMG20A Antibody (4D5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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