HMGA2 Antibody (763)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43640

Novus Biologicals, part of Bio-Techne
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NBP2-43640
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Predicted:

Chicken (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 763

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMGA2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (80%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMGA2 Antibody (763)

Western Blot: HMGA2 Antibody (763) [NBP2-43640]

Western Blot: HMGA2 Antibody (763) [NBP2-43640]

Western Blot: HMGA2 Antibody (763) [NBP2-43640] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [763] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: HMGA2 Antibody (763) [NBP2-43640]

Immunocytochemistry/ Immunofluorescence: HMGA2 Antibody (763) [NBP2-43640]

Immunocytochemistry/Immunofluorescence: HMGA2 Antibody (763) [NBP2-43640] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA2 protein stained by HMGA2 antibody [763] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640]

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640]

Immunohistochemistry-Paraffin: HMGA2 Antibody (763) [NBP2-43640] - Mouse brain. HMGA2 stained by HMGA2 antibody [763] (GTX629478) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for HMGA2 Antibody (763)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMGA2

HMGA2 encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

High Mobility Group AT-hook 2

Alternate Names

BABL, HMGIC, LIPO

Gene Symbol

HMGA2

Additional HMGA2 Products

Product Documents for HMGA2 Antibody (763)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGA2 Antibody (763)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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