HMGB2 Antibody (8P10V5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16774

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16774-100ul
NBP3-16774-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8P10V5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HMGB2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGB2 (P26583). MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDMAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMGB2 Antibody (8P10V5)

Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774]

Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774]

Western Blot: HMGB2 Antibody (8P10V5) [NBP3-16774] - Analysis of extracts of various cell lines, using HMGB2 Rabbit mAb (NBP3-16774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774]

Immunocytochemistry/ Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774]

Immunocytochemistry/Immunofluorescence: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunofluorescence analysis of U-2 OS cells using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774]

Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774]

Immunohistochemistry-Paraffin: HMGB2 Antibody (8P10V5) [NBP3-16774] - Immunohistochemistry of paraffin-embedded mouse testis using HMGB2 Rabbit mAb (NBP3-16774) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for HMGB2 Antibody (8P10V5)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HMGB2

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Alternate Names

High mobility group protein 2, high mobility group protein B2, high-mobility group box 2, HMG-2, HMG2high-mobility group (nonhistone chromosomal) protein 2

Gene Symbol

HMGB2

Additional HMGB2 Products

Product Documents for HMGB2 Antibody (8P10V5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HMGB2 Antibody (8P10V5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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