HMGB2 Antibody (S02-5I5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19876

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19876-100ul
NBP3-19876-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-5I5

Concentration

0.3 mg/ml

View all HMGB2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human HMGB2 (Uniprot # P26583)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMGB2 Antibody (S02-5I5)

Western Blot: HMGB2 Antibody (S02-5I5) [NBP3-19876]

Western Blot: HMGB2 Antibody (S02-5I5) [NBP3-19876]

Western Blot: HMGB2 Antibody (S02-5I5) [NBP3-19876] - Western blot detection of HMGB2 in CHO-K1, Ramos cell lysates using NBP3-19876 (1:1000 diluted). Predicted band size: 24kDa. Observed band size: 24kDa.
Immunohistochemistry-Paraffin: HMGB2 Antibody (S02-5I5) [NBP3-19876]

Immunohistochemistry-Paraffin: HMGB2 Antibody (S02-5I5) [NBP3-19876]

Immunohistochemistry-Paraffin: HMGB2 Antibody (S02-5I5) [NBP3-19876] - Immunohistochemistry of HMGB2 in paraffin-embedded Human tonsil using NBP3-19876 at dilution 1/50

Applications for HMGB2 Antibody (S02-5I5)

Application
Recommended Usage

Immunohistochemistry

1/50

Western Blot

1/2000-1/10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB2

HMGB2 encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.

Alternate Names

High mobility group protein 2, high mobility group protein B2, high-mobility group box 2, HMG-2, HMG2high-mobility group (nonhistone chromosomal) protein 2

Gene Symbol

HMGB2

Additional HMGB2 Products

Product Documents for HMGB2 Antibody (S02-5I5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HMGB2 Antibody (S02-5I5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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