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HMGB4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93780

Novus Biologicals, part of Bio-Techne
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NBP1-93780
NBP1-93780-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPSSFLLFCQDHYAQLKRENPNWSVVQVAKATGKMWSTATDLEKHPYEQRVALLRAKYFEELELYRKQCNARKKYRMS

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24309898)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HMGB4 Antibody

Western Blot: HMGB4 Antibody [NBP1-93780]

Western Blot: HMGB4 Antibody [NBP1-93780]

Western Blot: HMGB4 Antibody [NBP1-93780] - Analysis in control (vector only transfected HEK293T lysate) and HMGB4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780]

Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780]

Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining in human testis and endometrium tissues using anti-HMGB4 antibody. Corresponding HMGB4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780]

Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780]

Immunohistochemistry-Paraffin: HMGB4 Antibody [NBP1-93780] - Staining of human testis shows strong nuclear positivity in a subset of cells in seminiferus ducts.

Applications for HMGB4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HMGB4

Alternate Names

dJ1007G16.5, FLJ40388, high mobility group box 4, high mobility group protein B4, HMG2 like, MGC88128

Gene Symbol

HMGB4

Additional HMGB4 Products

Product Documents for HMGB4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HMGB4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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