HMGCS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16861

Novus Biologicals, part of Bio-Techne
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NBP2-16861
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HMGCS1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HMGCS1. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HMGCS1 Antibody

HMGCS1 Antibody

Western Blot: HMGCS1 Antibody [NBP2-16861] -

Western Blot: HMGCS1 Antibody [NBP2-16861] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HMGCS1 antibody (NBP2-16861) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: HMGCS1 Antibody [NBP2-16861]

Immunocytochemistry/ Immunofluorescence: HMGCS1 Antibody [NBP2-16861]

Immunocytochemistry/Immunofluorescence: HMGCS1 Antibody [NBP2-16861] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGCS1 protein stained by HMGCS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Scale bar = 10 um.
Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861]

Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861]

Immunohistochemistry-Paraffin: HMGCS1 Antibody [NBP2-16861] - Mouse liver. HMGCS1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Applications for HMGCS1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HMGCS1

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoAreductase

Alternate Names

3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble), 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble), EC 2.3.3.10, HMG-CoA synthase, HMGCS, hydroxymethylglutaryl-CoA synthase, cytoplasmic, MGC90332

Gene Symbol

HMGCS1

UniProt

Q01581 (Human)

Additional HMGCS1 Products

Product Documents for HMGCS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HMGCS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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