HMGCS2 Antibody (19C11)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26223
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 19C11
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HMGCS2 Antibody (19C11)
Immunogen
A synthesized peptide derived from Human HMGCS2 [UniProt P54868]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HMGCS2 Antibody (19C11)
Immunohistochemistry: HMGCS2 Antibody (19C11) [NBP3-26223] -
Immunohistochemistry: HMGCS2 Antibody (19C11) [NBP3-26223] - Image of HMGCS2 Antibody (19C11) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry: HMGCS2 Antibody (19C11) [NBP3-26223] -
Immunohistochemistry: HMGCS2 Antibody (19C11) [NBP3-26223] - Image of HMGCS2 Antibody (19C11) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Western Blot: HMGCS2 Antibody (19C11) [NBP3-26223] -
Western Blot: HMGCS2 Antibody (19C11) [NBP3-26223] - Positive Western Blot detected in: A549 whole cell lysate, Ntera-2 whole cell lysate.All lanes: HMGCS2 Antibody at 1: 500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 57, 53, 51 kDa
Observed band size: 55 kDa
Applications for HMGCS2 Antibody (19C11)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: HMGCS2
Alternate Names
3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial), 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial), EC 2.3.3.10, HMG-CoA synthase, hydroxymethylglutaryl-CoA synthase, mitochondrial, mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase
Gene Symbol
HMGCS2
Additional HMGCS2 Products
Product Documents for HMGCS2 Antibody (19C11)
Product Specific Notices for HMGCS2 Antibody (19C11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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