HMGCS2 Antibody (5T5T3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15696
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5T5T3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 409-508 of human HMGCS2 (P54868). AFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLASRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHRRKYARRPV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HMGCS2 Antibody (5T5T3)
![Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696]](https://resources.bio-techne.com/images/products/HMGCS2-Antibody-5T5T3-Western-Blot-NBP3-15696-img0002.jpg "Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696]")
Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696]
Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696] - Western blot analysis of extracts of various cell lines, using HMGCS2 antibody (NBP3-15696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry-Paraffin: HMGCS2 Antibody (5T5T3) [NBP3-15696]](https://resources.bio-techne.com/images/products/HMGCS2-Antibody-5T5T3-Immunohistochemistry-Paraffin-NBP3-15696-img0001.jpg "Immunohistochemistry-Paraffin: HMGCS2 Antibody (5T5T3) [NBP3-15696]")
Immunohistochemistry-Paraffin: HMGCS2 Antibody (5T5T3) [NBP3-15696]
Immunohistochemistry-Paraffin: HMGCS2 Antibody (5T5T3) [NBP3-15696] - Immunohistochemistry of paraffin-embedded human liver using HMGCS2 Rabbit mAb (NBP3-15696) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696]](https://resources.bio-techne.com/images/products/HMGCS2-Antibody-5T5T3-Western-Blot-NBP3-15696-img0003.jpg "Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696]")
Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696]
Western Blot: HMGCS2 Antibody (5T5T3) [NBP3-15696] - Western blot analysis of extracts of HepG2 cells, using HMGCS2 antibody (NBP3-15696) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Applications for HMGCS2 Antibody (5T5T3)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HMGCS2
Alternate Names
3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial), 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial), EC 2.3.3.10, HMG-CoA synthase, hydroxymethylglutaryl-CoA synthase, mitochondrial, mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase
Gene Symbol
HMGCS2
Additional HMGCS2 Products
Product Documents for HMGCS2 Antibody (5T5T3)
Product Specific Notices for HMGCS2 Antibody (5T5T3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...